常用荧光染料的激发及发射波长-友沃可

荧光素名称	激发波长(nm)	发射波长(nm)	备注
1,5IAEDANS	336	490
1,8-ANS	372	480
4-Methylumbelliferone	385	502
5-carboxy-2,7-dichlorofluorescein	504	529
5-Carboxyfluorescein(5-FAM)	492	518
5-Carboxynapthofluorescein(pH10)	512/598	563/668	RatioDye,pH
5-Carboxytetramethylrhodamine(5-TAMRA)	542	568
5-FAM(5-Carboxyfluorescein)	492	518
5-HAT(HydroxyTryptamine)	370-415	520-540
5-HydroxyTryptamine(HAT)	370-415	520-540
5-ROX(carboxy-X-rhodamine)	578 567	604 591
5-TAMRA(5-Carboxytetramethylrhodamine)	548 542	552 568
6-Carboxyrhodamine6G	518	543
6-CR6G	518	543
6-JOE	520	548
7-Amino-4-methylcoumarin	351	430
7-AminoactinomycinD(7-AAD)	546	647
7-Hydroxy-4-methylcoumarin	360	449,455
9-Amino-6-chloro-2-methoxyacridine	412,430	471,474
ABQ	344	445
AcidFuchsin	540	630
ACMA(9-Amino-6-chloro-2-methoxyacridine)	412,430	471,474
AcridineOrange+DNA	502	526
AcridineOrange+RNA	460	650
AcridineOrange,bothDNA&RNA	440-480	520-650
AcridineRed	455-600	560-680
AcridineYellow	470	550
Acriflavin	436	520
AcriflavinFeulgenSITSA	355-425	460
Aequorin(Photoprotein)		466	Photoprotein
AFPs-AutoFluorescentProtein-(QuantumBiotechnologies)seesgGFP,sgBFP
AlexaFluor350	346 342	442 441
AlexaFluor430	431	540
AlexaFluor488	495,492	519,520
AlexaFluor532	531,532	553,554
AlexaFluor546	556,557	572,573
AlexaFluor568	577,578	603
AlexaFluor594	590,594	617,618
AlexaFluor633	632	650
AlexaFluor647	647	666
AlexaFluor660	668	698
AlexaFluor680	679	702
AlizarinComplexon	530-560,580	580,624-645
AlizarinRed	530-560	580
Allophycocyanin(APC)	630,645	655,660
AMC,AMCA-S	345	445
AMCA(Aminomethylcoumarin)	345 347	425 444
AMCA-X	353	442
AminoactinomycinD	555	655
Aminocoumarin	346 350	442 445
Aminomethylcoumarin(AMCA)	345 347	425 444
AnilinBlue		600
Anthrocylstearate	360-381	446
APC(Allophycocyanin)	630,645	655,660
APC-Cy7	625-650	755
APTRA-BTC=RatioDye,Zn2+	466/380	520/530	RatioDye,Zn2+
APTS	424	505
AstrazonBrilliantRed4G	500	585
AstrazonOrangeR	470	540
AstrazonRed6B	520	595
AstrazonYellow7GLL	450	480
Atabrine	436	490
ATTO-TAGCBQCA	465	560
ATTO-TAGFQ	486	591
Auramine	460	550
AurophosphineG	450	580
Aurophosphine	450-490	515
BAO9(Bisaminophenyloxadiazole)	365	395
BCECF(highpH)	492,503	520,528
BCECF(lowpH)	482	520
BerberineSulphate	430	550
BetaLactamase	409	447,520
BFPblueshiftedGFP(Y66H) BlueFluorescentProtein	381,382,383	445,447,448	blueshiftedGFP(Y66H) BlueFluorescentProtein
BFP/GFPFRET
Bimane	398	490
Bisbenzamide	360	461
Bisbenzimide(Hoechst)	360	461
bis-BTC=RatioDye,Zn2+	455/405	529/505	RatioDye,Zn2+
BlancophorFFG	390	470
BlancophorSV	370	435
BOBO-1	462	481
BOBO-3	570	602
Bodipy492/515	490	515
Bodipy493/503	533	549
Bodipy500/510	509	515
Bodipy505/515	502	510
Bodipy530/550	528	547
Bodipy542/563	543	563
Bodipy558/568	558	569
Bodipy564/570	564	570
Bodipy576/589	579	590
Bodipy581/591	584	592
Bodipy630/650-X	625	642
Bodipy650/665-X	647	665
Bodipy665/676	605	676
BodipyFl	504,505	511,513
Bodipy FLATP	505	514
BodipyFl-Ceramide	505	511
BodipyR6GSE	528	547
BodipyTMR	542	574
BodipyTMR-Xconjugate	544	573
BodipyTMR-X,SE	544	570
BodipyTR	589	617
BodipyTRATP	591	620
BodipyTR-XSE	588	616
BO-PRO-1	462	481
BO-PRO-3	544	570
BrilliantSulphoflavinFF	430	520
BTC-RatioDyeCa2+	464/401	533/529	RatioDyeCa2+
BTC-5N-atioDye,Zn2+	459/417	517/532	RatioDye,Zn2+
Calcein	494	517
CalceinBlue	373	440
CalciumCrimson	588,589	611,615
CalciumGreen	501,506	531
CalciumGreen-1Ca2+Dye	506	531	Ca2+Dye
CalciumGreen-2Ca2+	506/503	536	Ca2+
CalciumGreen-5NCa2+	506	532	Ca2+
CalciumGreen-C18Ca2+	509	530	Ca2+
CalciumOrange	549	575 576
CalcofluorWhite	385,395,405	437,440,445
Carboxy-X-rhodamine(5-ROX)	576	601
CascadeBlue	377 398 399	420 423
CascadeYellow	399 400	550 552
Catecholamine	410	470
CCF2(GeneBlazer)
CFDA	494	520
CFP-CyanFluorescentProtein	430,433,436,(453)	474,475,476,(501)	CyanFluorescentProtein
CFP/YFPFRET
Chlorophyll	480	650
ChromomycinA	436-460	470
ChromomycinA	445	575
CL-NERF(RatioDye,pH)	504/514	540	RatioDye,pH
CMFDA	494	520
CoelenterazineCa2+Dye,bioluminescence	(429)	465	Ca2+Dye,bioluminescence, nativemolecule
Coelenterazinecp(Ca2+Dye,)	(430)	442	Ca2+Dye,bioluminescence
Coelenterazinef	(437)	473	Ca2+Dye,bioluminescence
Coelenterazinefcp		452	Ca2+Dye,bioluminescence
Coelenterazineh	(437)	464	Ca2+Dye,bioluminescence
Coelenterazinehcp	(433)	444	Ca2+Dye,bioluminescence
Coelenterazineip		441	Ca2+Dye,bioluminescence
Coelenterazinen	(431)	467	Ca2+Dye,bioluminescence
CoelenterazineO	460	575
CoumarinPhalloidin	387	470
C-phycocyanine
CPMMethylcoumarin	384	469	Methylcoumarin
CTC	400-450	602
CTCFormazan
Cy2	489	506
	554	568
	581	598
Cy3	514 552 554	566 570
	649	666
	675	695
Cy5	649	666 670
Cy7	710,743	767,805
CyanGFP	433(453)	475(501)
cyclicAMPFluorosensor(FiCRhR)	500	517
CyQuantCellProliferationAssay	480	520	CellProliferationAssay
Dabcyl	453
Dansyl	340	578
DansylAmine	337	517
DansylCadaverine	335	518
DansylChloride	372	518
DansylDHPE	336	517
Dansylfluoride	356	none
DAPI	359	461
Dapoxyl	403	580
Dapoxyl2	374	574
Dapoxyl3	373	574
DCFDA	504	529
DCFH(DichlorodihydrofluoresceinDiacetate)	505	535
DDAO	463	607
DHR(Dihydorhodamine123)	505	534
Di-4-ANEPPS	496	705
Di-8-ANEPPS(non-ratio)	488 498	605 713
DiA(4-Di-16-ASP)	456	591
DichlorodihydrofluoresceinDiacetate(DCFH)	505	535
DiD-LipophilicTracer	644	665	LipophilicTracer
DiD(DiIC18(5))	644	665
DIDS	341	415
Dihydorhodamine123(DHR)	505	535
DiI(DiIC18(3))	549,551	565
Dinitrophenol	349
DiO(DiOC18(3))	484,487	501,502
DiR	748	780	LipophilicTracer
DiR(DiIC18(7))	750	779
DM-NERF(highpH)	497/510	540	RatioDye,pH
DNP	349
Dopamine	340	490-520
DsRed	558	583	Redfluorescentprotein
DTAF	494	520
DY-630-NHS	621	660	Hemicyane labelforproteinsandDNA
DY-635-NHS	634	664	Hemicyane labelforproteinsandDNA
EBFP	383	447	EnhancedBlueFluorescentProtein
ECFP	436	474	EnhancedCyanFluorescentProtein
EGFP	488,498	507,516	EnhancedGreenFluorescentProtein
ELF97	345	530
Eosin	524	545
Erythrosin	529,532	554,555
ErythrosinITC	529	555
EthidiumBromide	510,523	595,605
Ethidiumhomodimer-1(EthD-1)	528	617
Euchrysin	430	540
EukoLight
Europium(III)chloride
EYFP	513,520	527,532	EnhancedYellowFluorescentProtein
FastBlue	360	440
FDA	494	520
Feulgen(Pararosaniline)	570	625
FIF(FormaldehydInducedFluorescence)	405	433
FITC	490,494	520,525
FITCAntibody	493	517
FlazoOrange	375-530	612
Fluo-3	480–506,506	520,527
Fluo-4	494	516
Fluorescein(FITC)	490,494	520,525
FluoresceinDiacetate	494	520
Fluoro-Emerald	495	524
Fluoro-Gold(Hydroxystilbamidine)	361	536
Fluor-Ruby	555	582
FluorX	494	520
FM1-43	479	598
FM4-46	515	640
FuraRed(highpH)	572	657
FuraRed/Fluo-3
Fura-2,highcalcium	335	505	Excitationratiodye
Fura-2,lowcalcium	363	512	Excitationratiodye
Fura-2/BCECF
GenacrylBrilliantRedB	520	590
GenacrylBrilliantYellow10GF	430	485
GenacrylPink3G	470	583
GenacrylYellow5GF	430	475
GeneBlazer(CCF2)
GFP(S65T)	498	516
GFPredshifted(rsGFP)	498	516
GFPwildtype,non-UVexcitation(wtGFP)	475	509
GFPwildtype,UVexcitation(wtGFP)	395	509
GFPuv	385	508
GloxalicAcid	405	460
GranularBlue	355	425
Haematoporphyrin	530-560	580
Hoechst33258	345	487
Hoechst33342	347	483
Hoechst34580	392	440
HPTS	355	465
Hydroxycoumarin	325-360	386-455
Hydroxystilbamidine(FluoroGold)	361	536
Hydroxytryptamine	400	530
Indo-1,highcalcium	330	401	Emissionratiodye
Indo-1,lowcalcium	346	475	Emissionratiodye
Indodicarbocyanine(DiD)	644	665
Indotricarbocyanine(DiR)	748	780
IntrawhiteCf	360	430
JC-1	514	529
JO-JO-1	530	545
JO-PRO-1	532	544
LaserPro	795	812
Laurodan	355	460
LDS751(DNA)	543	712
LDS751(RNA)	590	607
LeucophorPAF	370	430
LeucophorSF	380	465
LeucophorWS	395	465
LissamineRhodamine	572,577	591,592
LissamineRhodamineB	577	592
LIVE/DEADKitAnimalCells Calcein/Ethidiumhomodimer	494 528	517 617	formoredetailsrefer to
LOLO-1	566	580
LO-PRO-1	568	581
LuciferYellow	425,428	528,536,540
LysoTrackerBlue	373	422
LysoTrackerBlue-White	466	536
LysoTrackerGreen	504,534	511,551
LysoTrackerRed	490	516
LysoTrackerYellow	551	576
LysoSensorBlue	374	424
LysoSensorGreen	442	505
LysoSensorYellow/Blue	384	540
MagGreen	507	531
MagdalaRed(PhloxinB)	524	600
Mag-FuraRed	483/427	659/631	RatioDye,Mg2+
Mag-Fura-2	369/329 369/330	508 511/491	RatioDyeCa2+ RatioDyeMg2+
Mag-Fura-5	369/330 369/332	505/500 505/482	RatioDye,Ca2+ RatioDye,Mg2+
Mag-Indo-1	349/328 349/330	480/390 480/417	RatioDye,Ca2+ RatioDye,Mg2+
MagnesiumGreen	506,507	531
MagnesiumOrange	550	575
MalachiteGreen	628
MarinaBlue	362	459
MaxilonBrilliantFlavin10GFF	450	495
MaxilonBrilliantFlavin8GFF	460	495
Merocyanin	555	578
Methoxycoumarin	360	410
MitotrackerGreenFM	490	516
MitotrackerOrange	551	576
MitotrackerRed	578	599
Mitramycin	450	470
Monobromobimane	398	490
Monobromobimane(mBBr-GSH)	398	500
Monochlorobimane	380	461
MPS(MethylGreenPyronineStilbene)	364	395
NBD	466	539
NBDAmine	450	530
NileRed	515-555,559	590,640
Nitrobenzoxadidole	465	510-650
Noradrenaline	340	490-520
NuclearFastRed	289-530	580
NuclearYellow	365	495
NylosanBrilliantIavinE8G	460	510
OregonGreen	503	522
OregonGreen488-X	494	517
OregonGreen	503	522
OregonGreen488	490,493	514,520
OregonGreen500	497	517
OregonGreen514	506	526
PacificBlue	405	455
Pararosaniline(Feulgen)	570	625
PBFI	340/380	420	Excitationratiodye
PE-Cy5	488	670
PE-Cy7	488	755,767
PerCP	488	675
	488	710
PE-TexasRed[Red613]	488	613
PhloxinB(MagdalaRed)	524	600
Phorwite AR	360	430
PhorwiteBKL	370	430
PhorwiteRev	380	430
PhorwiteRPA	375	430
Phosphine3R	465	565
PhotoResist	365	610
PhycoerythrinB[PE]	546-565	575
PhycoerythrinR[PE]	565	578
PKH26(Sigma)	551	567
PKH67	496	520	Chroma
PMIA	341	376
PontochromeBlueBlack	535-553	605
POPO-1	433	457
POPO-3	533	574
PO-PRO-1	435	455
PO-PRO-3	539	567
Primuline	410	550
ProcionYellow	470	600
PropidiumIodid(PI)	(305),536,538	617
PyMPO	412,415	561,564,570
Pyrene	360	387
Pyronine	410	540
PyronineB	540-590	560-650
PyrozalBrilliantFlavin7GF	365	495
QSY7	560
QuinacrineMustard	440	510
Red613[PE-TexasRed]	488	613
Resorufin	571	584,585
RH414	532	716
Rhod-2	552	576
Rhodamine	550	573
Rhodamine110	496,497	520
Rhodamine123	507	529
Rhodamine5GLD	470	565
Rhodamine6G	525	555
RhodamineB	540	625
RhodamineB200	523-557	595
RhodamineBextra	550	605
RhodamineBB	540	580
RhodamineBG	540	572
RhodamineGreen	502	527
RhodaminePhallicidine	558,542	575,565
RhodaminePhalloidine	542	565
RhodamineRed	570	590
RhodamineWT	530	555
RoseBengal	525,540	550-600
R-phycocyanine
R-phycoerythrin(PE)	565	578
rsGFP	473	509	redshiftedGFP(S65T)
S65A	471	504
S65C	479	507
S65L	484	510
S65T	488	511
SapphireGFP	395	511
SBFI	340/380	420	Excitationratiodye
Serotonin	365	520-540
SevronBrilliantRed2B	520	595
SevronBrilliantRed4G	500	583
SevronBrilliantRedB	530	590
SevronOrange	440	530
SevronYellowL	430	490
sgBFP	387	450
sgBFP(superglowBFP)	387	450	Quantum’sSuperGloGFP AFPs
sgGFP	474	488
sgGFP(superglowGFP)	474	509	Quantum’sSuperGloGFP AFPs
SITS	336	436	IonChannels
SITS(Primuline)	395-425	450
SITS(StilbeneIsothiosulphonicAcid)	365	460
SNAFLcalcein	506/535	535/620	RatioDye,pH
SNAFL-1	508/540	543/623	RatioDye,pH
SNAFL-2	514/543	546/630	RatioDye,pH
SNARFcalcein	552/574	590/629	RatioDye,pH
SNARF1	576/548	635/587	Excitationandemissionratiodye
SodiumGreen	506,507	532	Na+,K+
SpectrumAqua	433,/53	480/55	Vysis
SpectrumGreen	497/30,509/31	538/44,524/56	Vysis
SpectrumOrange	559/38,560	588/48	Vysis
SpectrumRed	587,587/35	612,612/51
SPQ(6-methoxy-N-(3-sulfopropyl)quinolinium)	344	443
Stilbene	335	440
SulphorhodamineBcanC	520	595
SulphorhodamineGExtra	470	570
SYTO11	508,510	527,530	DyeforDNA,RNA
SYTO12	499,500	522,519	DyeforDNA,RNA
SYTO13	488,491	509,514	DyeforDNA,RNA
SYTO14	517,521	549,547	DyeforDNA,RNA
SYTO15	516,518	546,555	DyeforDNA,RNA
SYTO16	488,494	518,525	DyeforDNA,RNA
SYTO17	621	634	DyeforDNA
SYTO18	490,493	507,527	DyeforDNA,RNA
SYTO20	512	530	DyeforDNA
SYTO21	494	517	DyeforDNA
SYTO22	515	535	DyeforDNA
SYTO23	499	520	DyeforDNA
SYTO24	490	515	DyeforDNA
SYTO25	521	556	DyeforDNA
SYTO40	420	441	DyeforDNA
SYTO41	430	454	DyeforDNA
SYTO42	433	460	DyeforDNA
SYTO43	436	467	DyeforDNA
SYTO44	446	471	DyeforDNA
SYTO45	452	484	DyeforDNA
SYTO59	622	645	DyeforDNA
SYTO60	652	678	DyeforDNA
SYTO61	628	645	DyeforDNA
SYTO62	652	676	DyeforDNA
SYTO63	657	673	DyeforDNA
SYTO64	599	619	DyeforDNA
SYTO80	531	545	NucleicAcidStain
SYTO81	530	544	NucleicAcidStain
SYTO82	541	560	NucleicAcidStain
SYTO83	543	559	NucleicAcidStain
SYTO84	567	582	NucleicAcidStain
SYTO85	567	583	NucleicAcidStain
SYTOXBlue	445	470	NucleicAcidStain
SYTOXGreen	504	523	NucleicAcidStain
SYTOXOrange	547	570	NucleicAcidStain
Tetracycline	390-425	525-560
Tetramethylrhodamine(TRITC)	555	576
TexasRed	595	620
TexasRed-Xconjugate	595	615
Thiadicarbocyanine(DiSC3)	651,653	674,675
ThiazineRedR	596	615
ThiazoleOrange	510	530
Thioflavin5	430	550
ThioflavinS	430	550
ThioflavinTCN	350	460
Thiolyte	370-385	477-488
ThiozoleOrange	453	480
TinopolCBS(CalcofluorWhite)	390	430
TMR	550	573
TO-PRO-1	515	531
TO-PRO-3	644	657
TO-PRO-5	747	770
TOTO-1	514	531,533
TOTO-3	642	660
TriColor(PE-Cy5)	(488)650	667
TRITCTetramethylRodamineIsoThioCyanate	550	573
TrueBlue	365	425
TruRed	490	695
Ultralite	656	678
UranineB	420	520
UvitexSFC	365	435
wtGFP	395(475)	508	wildtypeGFP
WW781	605	639
X-Rhodamine	580	605
XRITC	582	601
XyleneOrange	546	580
Y66F	360	508
Y66H	360	442
Y66W	436	485
YellowGFP	513	527	Yellowshifted GreenFluorescentProtein
YFP	513,520	527,532	YellowFluorescentProtein
YO-PRO-1	491	506
YO-PRO-3	613	629
YOYO-1	491	508,509
YOYO-3	612	631